DNA

Part:BBa_K1015004:Design

Designed by: Kento Tominaga   Group: iGEM13_TMU-Tokyo   (2013-08-29)


Escherichia coli araC gene (for RED system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1028
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1010


Design Notes

For target site of homologous recombination with E.coli genome araC , using RED recombination system.

Source

Escherichia coli

References